Can You Take Melatonin and Vitamin B6 Together?

Dose or timing may matter

The short answer

Vitamin B6 is a cofactor for aromatic amino acid decarboxylase, which converts 5-HTP to serotonin (a melatonin precursor). High-dose B6 may increase endogenous melatonin production, creating additive sedation with supplemental melatonin.

How to arrange timing

If taking both, start with lower melatonin doses as B6 may enhance endogenous melatonin production.

Reference

Reference noted for this combination: Ebadi M et al. Aminoalkyl-induced convulsions and the changes in biogenic amines and their pyridoxal phosphate-dependent enzymes. Neurochem Res. 1980;5(9):999-1011

Important context

Whether supplements suit you together also depends on dose, product formulation, health conditions, and medication use. Ask a doctor or pharmacist first if you take medication, are pregnant or breastfeeding, or have a chronic condition.