Can You Take L-Proline and Vitamin C Together?

May affect absorption

The short answer

Vitamin C is essential for the hydroxylation of proline to hydroxyproline in collagen synthesis. While they work synergistically in collagen production, high-dose Vitamin C may alter proline transporter kinetics when taken simultaneously.

How to arrange timing

While L-Proline and Vitamin C are synergistic for collagen synthesis, separate by 30 minutes for optimal individual absorption.

Reference

Reference noted for this combination: Peterkofsky B. Ascorbate requirement for hydroxylation and secretion of procollagen. Am J Clin Nutr. 1991;54(6 Suppl):1135S-1140S.

Important context

Whether supplements suit you together also depends on dose, product formulation, health conditions, and medication use. Ask a doctor or pharmacist first if you take medication, are pregnant or breastfeeding, or have a chronic condition.